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Future research must therefore embrace greater ecological complexity, moving beyond simplified laboratory assays towards mesocosm experiments and ecosystem function endpoints to probe these subtle but potentially significant environmental impacts

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Then 1 ml of the -galactosidase staining solution was added to each well, and incubated at 37 C overnight

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Oocytes represent terminally differentiated, non-renewable germ cells that rely extensively on mitochondrial oxidative phosphorylation (OXPHOS) during maturation, fertilization, and early embryonic development [29, 30]