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Improved Long-Term Outcome with Chemoradiotherapy Strategies in Locoregionally Advanced Esophageal Cancer

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Immunoprecipitation (IP) and Co-immunoprecipitation (Co-IP) assay Cells or tissues were lysed in IP lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.5% Triton X-100, 0.5% NP-40, 1 mM EDTA, and 1 mM EGTA, 1 mM -glycerophosphate, 1 mM PMSF, and 1X PIC)

This project was supported by Khalifa Center for Genetic Engineering and Biotechnology-UAEU (Grant number: 12R255), and the UAEU program of Advanced Research (Grant number: 12S169)

When MG001 was incubated with 80 mg/L l-cysteine for 2 h, the residual amount of l-cysteine was 65.76 1.95 mg/L (82.2 0.02 % of the initial amount)