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Western blot analysis The analysis was performed as previously described 18

SAC administration increased Nrf2 and GPX4 expression while reducing Keap1 levels

This initial oxidation of the Cys thiol group to a sulfinic acid group is carried out by cysteine dioxygenase (CDO), which adds molecular oxygen to the sulfur of Cys, forming cysteinesulfinate [also called cysteinesulfinic acid (CSA) or 3-sulfinoalanine]

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Research has shown that PCs display chemopreventive properties in cancer cells by enhancing apoptosis and suppressing proliferation (Ldia et al