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& Davie, J

RNA Extraction and Real-Time Polymerase Chain Reaction Assay Total RNA was extracted from frozen jejunum samples with TRIzol reagent (Sigma-Aldrich), and was used to synthesize cDNA with Prime-Script RT reagent kit (Takara, Dalian, China) according to the manufacturers instructions

However, L-ca 200 significantly enhanced the expression levels of Bcl-2 compared to the MSG group

Primary infection was considered less likely given the absence of systemic symptoms and persistence despite systemic antibiotics, although secondary impetiginization could not be excluded