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Western blots were performed after running the samples in SDS-PAGE gels with Kaleidoscope (Bio-rad) or PageRuler Prestained Protein Ladder (Thermo Scientific) and transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-rad)

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Copper ions can catalyze the generation of reactive oxygen species, and copper homeostasis is maintained by a dedicated system of transporters, chaperones, and binding proteins (such as ceruloplasmin and albumin) that keep essentially no copper floating around unbound

NAC is a powerful amino acid that serves as the direct precursor to glutathione, the bodys master antioxidant, which neutralizes free radicals, supports liver detoxification, boosts immune function, and protects cells from oxidative stress