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McAfee et al ., 2010)

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This results is in agreement with several studies demonstrated that MTX induces oxidative stress in tissues companied with decreased antioxidants levels [38, 69]

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However, tandem mass spectrometry with electrospray ionisation has been used to measure TMA and TMAO following derivatisation of trimethylamine with ethyl bromoacetate to form ethyl betaine bromide