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Detection of Antioxidant Enzyme Activities Ascorbate peroxidase activity was detected as described in Dehydroascorbate reductase (DHAR) activity was determined as described in Glutathione reductase activity was detected as described in Preparation of Nonprotein Thiol Extracts The alga (0.2 g of DT) was frozen in liquid nitrogen and homogenized in a mortar with a pestle and extracted by addition of 1.2 ml of 0.1% (w/v) trifluoroacetic acid (TFA)6.3 mM diethylenetriaminepentaacetic acid (DTPA)

Qualitative and quantitative determination of ten major saponins in Platycodi Radix by high performance liquid chromatography with evaporative light scattering detection and mass spectrometry

The study utilized HA with different molecular weightslow molecular weight (LMW) and high molecular weight (HMW)due to their presumed distinct biological activities

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Superoxide combines with NO to form peroxynitrite that oxidizes BH 4 and destabilizes eNOS to produce more superoxide 22, 24, 25 thus, further enhancing the development of oxidative stress