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doi: 10.1007/s12020-020-02502-2 175 HlisnkovHPetroviovIKolenaBidlovskMSirotkinA

In vitro liquid-liquid phase transition assay 16-well Culture-Well chamber slips (Grace Bio-Labs) or 384-well plates (Cellvis, P384-1.5H-N), pre-treated with 5% (w/v) Pluronic F-127 (Sigma, P2443) overnight, were used to study in vitro phase separation

In vitro experiments have shown the formation of the reactive metabolite NAPQI and N-acetyl-p-benzosemiquinone imine (NAPSQI) by prostaglandin H 2 synthases (PTGS) [38,39]

A meta-analysis of studies investigating the effect of prebiotics on renal function revealed that supplementing with fiber markedly reduced serum urea levels