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PCR confirmed that the GR gene (1356 bp) had been replaced with a kanamycin resistance cassette (816 bp), and western blot analyses confirmed that the GR protein was no longer expressed in the mutant strain (Figures 1B and C)

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An in vivo and in vitro study on the protective effects of N-acetylcysteine on mitochondrial dysfunction in isoproterenol treated myocardial infarcted rats