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The cells were sub-cultured, when the cells are 80% confluent, using a cell scraper (AGC Techno Glass Co., Ltd) after processing by DPBS() (no calcium, no magnesium, Thermo Fisher Scientific Inc.) and CTK solution

Telomerase activation is common in cancer, while excessively short telomeres can also be harmful

The FSC results in the low scanning rate range agree well with the DSC results and the literature data

Purity is critical because contaminants can produce unintended biological effects, skewing data and invalidating results

186,187,509,510 The accumulated DNA oxidative damage in AD hyper-activates the DNA damage sensor PARP-1, thereby reducing the concentration of cellular NAD + and suppressing the function of NAD + -SIRT1-PGC-1 axis, which in turn causes abnormal mitochondria