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A sensible experiment spreads doses across at least three orders of magnitude, something like 1, 10, and 100 nanomolar on up to 1 and 10 micromolar, precisely because the sweet spot moves with the copper-to-peptide ratio, the buffer, and how much serum sits in the media

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Prior to introducing the compound into your laboratory workflow, reconstitution must be carried out using an appropriate sterile, non-pyrogenic, or bacteriostatic diluent