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This is biology, and it applies regardless of age

To inhibit mitochondrial metabolism and GSH synthesis, the following chemicals were purchased from Sigma: metformin, rotenone, antimycin A, oligomycin A and BSO

Statistical analyses Statistical analyses of the quantified data were performed by analysis of variance (ANOVA)

[DOI] [PMC free article] [PubMed] [Google Scholar] 8.Abbas H.K

Beger RD, Goodacre R, Jones CM, Lippa KA, Mayboroda OA et al (2024) Analysis types and quantification methods applied in UHPLC-MS metabolomics research: a tutorial