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Western Blot Analysis Kidney samples were homogenized in RIPA buffer with a protease inhibitor mix (complete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets, Roche, Basel, Switzerland), employing the TissueLyser LT to promote lysis

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This action of CHAC1 aligns with its regulation by transcription factors ATF4 and ATF3, suggesting a tightly controlled mechanism whereby CHAC1 contributes to the cellular apoptosis process, particularly in stress responses ( 4.5 Ferroptosis Ferroptosis is a regulated form of cell death characterized by the accumulation of lipid peroxides, particularly in membrane phospholipids, which occurs when the cellular antioxidant defense is insufficient