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The amount of plasmid DNA used for the transfection was 2 g for pcDNA3.1 + and pCMV-Gg PRX4 (encoding PRX4-FLAG) (10), and 6 g for pES104 (encoding GPx7-FLAG), and 6 g for pES105 (encoding full-length GPx8-FLAG)

Since reduced glutathione has a half-life of around 9 hours at pH 7.5 in the absence of metals (Stevens et al., 1983) and multiple times faster in the presence of certain metals (Voegtlin et al., 1931), we wanted to exclude that GSH oxidizes over time in our assay, resulting in the formation of GSSG

Among the inhibitors of GCL, l-buthionine sulfoximine (BSO) is the most potent and has already been in the clinical trial stage [11, 12]

I., Siltelioglu Turan, I., Mammadov, R., Altnkaynak, K., & Kisaoglu, A

Also, we obtained GPX7 gene expression profiling from three therapeutically relevant transcriptome microarrays: (i) GSE43452 dataset 36 , from a human GBM cell line (U87MG) treated with temozolomide at 20 M for 24 h, based on the Illumina GPL10558 (HumanHT-12 V4.0 expression beadchip)