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In order to improve outcomes, biphasic scaffolds can be created with a softer upper layer containing chondrogenic factors (e.g., TGF-, chondroitin sulfate) and a stiffer lower layer, often loaded with calcium phosphate, hydroxyapatite, or bone morphogenetic protein (BMP), to provide structural support of the above cartilage layer and to promote osteogenic tissue formation and boney integration

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GHK-Cu contributes by attracting stem cells, supporting vascular networks, and reducing oxidative damagekey mechanisms relevant to long-term musculoskeletal recovery [14]

Compare with Experimental Data : If possible, compare your estimated pKa value with experimental data