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Clinical improvements are typically observed within 812 weeks, with optimal results seen over 36 months depending on severity and compliance

The thrombin cleaved protein was purified by size-exclusion chromatography on a superdex-200 10/300 increase column (GE Life Sciences) in 4 mM decyl d-maltoside (Anatrace), 0.3 mM CHS, 0.001% (w/v) 1-pamitoyl-2-oleoyl- sn -glycero-3-phospho ethanolamine (Avanti Polar Lipids), 20 mM Tris-Cl (pH 8.0), 300 mM NaCl, and 5% glycerol

doi: 10.3390/molecules29092003 20 AssadR.ReshiZ

So they switched them onto the next approach

After 48 hours the discs are removed, the skin is examined and any redness or swellings are noted