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doi: 10.3390/nu10081122

One important caveat: doses above 40 mg/day exceed the tolerable upper intake level and should only be taken under medical supervision, as high-dose zinc can inhibit copper absorption and cause gastrointestinal effects

The CellTiter 96 Aqueous One Solution Cell Proliferation (MTS) assay was used to assess the extracts cytotoxic effect on MCF7 cells

Regulation of thiol-disuphide status has traditionally been associated with TRX (Buchanan & Balmer 2005).However, genetic support for the physiological importance of glutathione-dependent disulphide reduction comes from the observations that the NADPH-TRX and glutathione systems play overlapping roles in development (Reichheld et al