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Then, HHDPCs were washed twice with preheated PBS and the total RNA was extracted from cells using Trizol reagent (Invitrogen, USA)

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Fibroblast pyruvate carboxylase is required for collagen production in the tumour microenvironment

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Akt activates the IKK gene (CHUK gene) The arrow indicates that Akt phosphorylates the protein IKK, thereby activating the protein

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