Possible Cause: Complexes not thoroughly mixed in growth medium Suggested Solution: Following addition of transfection complexes into medium, ensure that the plate or wells are thoroughly mixed to prevent concentration of DNA:transfection reagent complexes in the wells Possible Cause: Cells have changed over time, or splitting conditions have changed Suggested Solution: If transfection performance suddenly declines, it may be because of the cells
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue sections (5 m) were used for haematoxylin and eosin staining and immunohistochemical analyses
PMID: 19649216, PMCID: PMC2719153
As an independent pathological entity, hyperuricemia extends beyond gout pathogenesis to contribute to systemic comorbidities, including atherosclerosis, chronic kidney disease, and metabolic syndrome
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