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Deoxycholate has a critical micelle concentration (CMC) that can vary based on temperature, but the concentration we used (1 mM) is below the CMC and can be removed by dialysis 34

The results in this study revealed that ILF3 was a key transcription factor that regulated DEPTOR, an important repressor in the mTOR pathway, highlighting the importance of the role of ILF3 in regulating mTOR signaling

Ali MY, Oliva CR, Noman ASM, Allen BG, Goswami PC, Zakharia Y, et al

JZP-458 was well tolerated in phase I clinical trials

however, the relative contribution of each is still unknown and calls for carefully planned clinical trials that consider weight fluctuations