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Description

The ubiquitinated forms of KCNN3, as well as the immunoprecipitated pull-down proteins, were detected by western blotting using anti-HA antibody, anti-ubiquitin antibody, or anti-KCNN3 antibody coupled with other labeling antibodies

The measurements were performed with a CytoFlex flow cytometer (Beckman Coulter Inc, Brea, California, USA) using the CytExpert program

As AIF deficiency potently compromises OXPHOS [32, 34], it is reasonable to speculate that AIF might be another target by which HIF downregulates mitochondrial respiration

Glutathione is the bodys master antioxidant a tripeptide synthesized in every cell that neutralizes reactive oxygen species, drives Phase II hepatic detoxification, supports immune function, and protects mitochondrial integrity