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Vitamin B12 is important in the maintenance of our metabolism, blood cells, and nerve function

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Subsequently, cells were washed with PBS and pre-extracted in CSK buffer [25 mM HEPES (pH 7.7), 50 mM NaCl, 1 mM EDTA, 3 mM MgCl 2 , 300 mM sucrose, 0.5% (v/v) Triton X-100] for 10 min at RT