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Several benefits of the established GSH affinity chromatography method were demonstrated in this work

The observed aggregates likely result from the inherent stickiness of Syn 1103 , wherein the exposure of its hydrophobic region slows diffusion and promotes promiscuous interactions with various cellular proteins, thereby interfering with their normal functions

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Skeletal muscle membrane preparation Wild-type RyR1 was purified from rabbit skeletal muscle (purchased from Pel-Freeze Biologicals), and R615C RyR1 was purified from muscle from pigs homozygous for this mutant (purchased from Boyle Farms, Moorehead, Iowa) as described before 38