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( I ) Ratio of GSR/GPX4 at protein level by Western blot was calculated in control and A2ARi-treated groups

However, compound 34 with a 5-chloro-2-fluoro substituted phenyl ring displayed the highest EC 50 in the RNA reduction-based assay and had considerably higher EC 50 in the CPE-based assay compared to other halide-substituted ebselen diselenide derivatives ( 25 , 27 , 30 )