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Description

The protocol is characterized by several distinct steps including two washes with 200 l of 8M UREA (8 M UA, 100 mM Tris-HCl buffer pH 8.0) and subsequent centrifugation at 14,000 g for 20 min at room temperature, followed by the addition of 50 l of 50 mM Iodioacetamide (IAA) and centrifugation at 6,000 g for 20 min at room temperature

The increase in glutathione abundance in infested resistant plants is at least partially achieved by increased synthesis via increased enzymatic activity and transcript abundance of genes encoding glutathione synthetases