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Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders MDCK cells were grown to 100% confluency on the coverslips in six-well plates and washed twice with PBS

While these findings come from animal and cell culture models rather than human clinical trials, the consistency of the respiratory protection data across multiple study designs and injury types is compelling

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Other peptides, such as TB-500 and GHK-Cu, are also used to support connective tissue healing and may be considered as part of a comprehensive regenerative approach

It differs from Ang (17) in only one amino acid in the N-terminal region