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Briefly, after the cells were treated with the test compound or the dissolving vehicle for 45 min, they were washed with ice-cold PBS, harvested by trypsinization, centrifuged, and re-suspended in PBS supplemented with the complete protease inhibitor cocktail and the same concentrations of the test compound

Luteolin isolated from the flowers of Lonicera japonica suppresses inflammatory mediator release by blocking NF-kappaB and MAPKs activation pathways in HMC-1 cells

Mizoue T, Inoue M, Tanaka K, Tsuji I, Wakai K, Nagata C, Tsugane S (2006) Tobacco smoking and colorectal cancer risk: an evaluation based on a systematic review of epidemiologic evidence among the Japanese population

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2021), and this conclusion was confirmed by the significant reduction of lipofuscin accumulation in C