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Then, the RAW264.7 cells were lysed using RIPA lysis buffer supplemented with 1 mM phenylmethanesulfonyl fluoride (PMSF)

Stearoyl-CoA desaturase 1 (SCD1), the rate-limiting enzyme in MUFA synthesis, enhances cellular resistance to ferroptosis by modulating the PUFA/MUFA ratio (28)

When the cellular activity promoting ferroptosis significantly exceeds the antioxidant buffering capacity provided by the ferroptosis defense system, there is an excessive accumulation of lipid peroxides on the cell membrane and subsequent membrane rupture, leading to cellular ferroptosis (Stockwell et al., 2020