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doi: 10.1042/BST20150108 38 LetunicI.BorkP

105 ScarffeL

Biochemistry (Mosc) 85, 15431553

( B ) HEK293T cells transiently expressing nsp14-FLAG were treated with the indicated concentrations of JC19 or NB001 for 0.5 h or EN450 for 3 h, and the insoluble lysate was analyzed by immunoblot

X-ray crystallography was used to investigate this complex enzymatic process, which showed Orf1 has two substrate-binding sites that sit 13.5 apart unlike canonical FAD-dependent oxidoreductases