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Successful mutants that amplified all hygromycin resistance cassette, left arm crossover region, and right arm crossover regions were plated on a fresh 7H11 plate containing 75 g/ml hygromycin

Ageing: is there a role for arachidonic acid and other bioactive lipids

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The lower pH protonates key amino acid residues especially histidine and glutamate which changes the peptide's electrical charge and stops molecules from sticking together