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J Biol Chem 244(22):60496055 McGuinness AJ, Sapey E (2017) Oxidative stress in COPD: sources, markers, and potential mechanisms

Cell viability assay Cells were enumerated by Trypan blue staining using a hemocytometer (Qiujing, Shanghai, China), then were seeded into 96-well plates at the density of 30004000 cells/well

We observed a 1:1:1 characterized singlet oxygen three-line spectrum in the CDs solution after 5 min of light stimulation, and the intensity of the spectrum was enhanced with the increase of CDs concentrations (Figure 3B)

Secondary amines are converted into lactam