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SKU: 63268529751
Price: 22.36 USD
Availability: InStock
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10.1155/2020/7627934 6 BaeY

doi: 10.1111/jns.12307 164 MillecampsS.JulienJ

After culture for 40 h, cells were collected and lysed in 8 ml of hypotonic buffer (10 mM HEPES pH 8.0, 10 mM KOAc, 1.5 mM Mg(OAc) 2 , 2% (v/v) glycerol, 5 mM NDSB-256 and 0.5 mM TCEP, with 1 tablet of cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001) per 10 ml of buffer) by a Dounce homogenizer