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Accumulated evidence indicated that patients with high microsatellite instability (MSI-H) are more likely to respond to immune checkpoint inhibition and benefit from immunotherapy 24,25

Eur J Cancer (2013) 49:25463

Western blot analysis of STING expression CH157, IOMM-LEE, MGS1, GBM12, and 0827 cell pellets stored at -80 C were thawed on ice, resuspended in cell lysis buffer (RIPA with 1X Halt protease inhibitor) and sonicated with a QSonica Q800R3 sonicator at 50% amplitude, 20 seconds on, 20 seconds off for a total of 40 seconds