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Spontaneous urination samples were collected during handling prior to injections on each treatment day and before euthanization

The filtrated protein was applied to a Superdex, 26/60, 75 pg (Cytiva, Freiburg, Germany) size-exclusion chromatography column pre-equilibrated in phosphate-buffered saline (PBS: 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , pH 7.4)

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