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Primary antibodies were diluted in TBST skimmed milk 5% buffer and the incubation was performed overnight at 4C using an orbital shaker followed by 2 h at room temperature with the secondary horseradish peroxidase (HRP)-linked antibody (Goat Anti-Mouse or Anti-Rabbit IgG Peroxidase Conjugated by Thermo Scientific)

[64] Of course, artifacts of cell culturee.g., removal of neurons from the influence of gliacould create spurious results as well

E.vanden HovenR

This demonstrates utility for bone tissue engineering applications, potentially addressing osteoporosis and bone healing challenges

Ongoing research points to a range of potential applications related to tissue support, inflammatory modulation, and overall well-being