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Collectively, these results indicate that L-theanine substantially strengthens systemic antioxidative defenses

For GSSG competition assay (Extended Data Figure 4l), the 2x reaction buffer consisted of 10LM [ 3 H]GSSG, 5mM GSSG, 150mM KCl, and 50mM HEPES (pH 7.4)

The binding of MT1, MT2, and glutathione (GSH) to Cu can limit the cytotoxicity of excess Cu

The colored end-product of the assay demonstrated remarkable stability at room temperature, with the absorbance at 412 nm remaining stable for over three hours

To overcome these issues, combining therapy with other inhibitors targeting molecules in the downstream pathways or immunotherapy is under investigation with great potential for patients with KRAS-driven cancers