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TIP3P water molecules 49 were added to the simulation box (final dimensions 126 x 126 x 186 ) using the VMD Solvate plugin 1.5 (VMD Solvate plugin, Version 1.5

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Western blotting dPC12 cells (5 10 4 cells/per well) were treated as described above and after incubation the cells were harvested and suspended in lysis buffer (10 mM Tris, 1 mM EDTA, 1% SDS, pH 7.5)