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The compound then received GRAS (Generally Recognized as Safe) designation from the FDA for use as a food ingredient, which is a separate regulatory category from drug approval

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Data that remained non-normally distributed and/or heteroscedastic after log transformation were analyzed (not log-transformed, but still 1e13-transformed) with a KruskalWallis test, while normally distributed and homoscedastic data were analyzed with a one-way ANOVA, performed on the log-transformed data as necessary

Specific oligonucleotide primers for 3-AR and actin were used, generating PCR products of 234 base pairs and 559 base pairs, respectively