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"Chronic oxidative damage together with genome repair deficiency in the neurons is a double whammy for neurodegeneration: Is damage response signaling a potential therapeutic target?"

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Cells were washed twice in ice-cold PBS and lysed for 15 min on ice with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with Benzonase (25 U per ml) and 1 Halt protease inhibitor cocktail