glutathione sepharose 4b pharmacia tesseract Glutathione 4B, 10 mL Suitable for batch GST tagged protein purification and scale Full article: YPXL/I Is a

Zhou, M., Wang, H., Shang, Y., Li, Y

Orexin receptor expression in human adiposa tissue: effects of orexin A and orexin B

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After measuring the concentration of the extracted protein utilizing a BCA protein assay kit (Beyotime), 20 g of extracted protein were separated by 10% SDS-PAGE gel electrophoresis and subsequently transferred onto 0.22 m polyvinylidene difluoride (PVDF, Millipore, USA) membranes