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The mRNA relative quantity of target genes was obtained from the method of comparative threshold cycle (CT) and the target gene level of expression were normalised to the -actin levels as an endogenous control within each group

Materials and methods Chemicals and reagents Span 60 (sorbitan monostearate), sodium deoxycholate (SDC), and dialysis membrane (molecular weight 12,00014,000 Da cutoff) were purchased from Sigma (St

This stage plays a critical role in determining the systemic exposure of TCM components, the duration of therapeutic effects, and the risk of toxicity

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Stable for up to 24 months when properly stored