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The RQ values were then used to graph relative gene expression over at least three replicate experiments using GraphPad Prism 8.0 ( Healthspan: body bends/movement in liquid media/thrashing/swimming Synchronized L4 stage animals were transferred to treatment plates and incubated at 20 C for 1, 3, 6, or 12 days

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Briefly, 50100 l of saturated culture was spun down and cells were thoroughly resuspended in 100200 l of lysing buffer (62.5 mM Tris-HCl, pH6.8, 10% glycerol, 2% SDS, 4% -mercaptoethanol)

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