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These effects were mediated through downstream activation of transcription factors including c-Fos, c-Jun, and EGR-1, which regulate genes involved in cell cycle progression, extracellular matrix remodelling, and angiogenic signalling

Cells in the HG condition were cultured in the medium containing 35 mM of D-glucose (Sigma-Aldrich), and cells in the SG condition were cultured in DMEM/F12 (this represents control, CTR, condition)

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GHK-Cu attracts immune cells (macrophages, mast cells) and endothelial cells to injury sites, stimulates angiogenesis (new blood vessel formation), and promotes nerve outgrowth

Campbell JD, et al