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After incubation in the dark for 20 min with gentle shaking to ensure effective probe uptake, the samples were incubated at 37C

Fluctuations in intraocular pressure (IOP), insufficient blood supply to the optic nerve, malnutrition, trauma, hypoxia-induced inflammatory responses, and degeneration of RGCs are the basic factors for its onset (123), among which the increase in pathological intraocular pressure (ph-IOP) and the death of RGCs are the key factors (124)

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In essence, bremelanotide can fill a gap for those who cannot use or tolerate the first-line oral medications

Results vary and should always be supervised by a medical professional