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Some patients report a warming or tingling sensation during procedures, but these effects are temporary and mild

Much depends on the post-mortem interval, the amount of trauma to the body and addition of fluoride ions as an enzyme inhibitor and preservative and femoral or cardiac blood was taken for analysis [45-47]

Luciferin detection reagent (LDR) (100 l) was added to all wells, briefly shaken and incubated for 15 min at room temperature and the luminescence measured using microplate imager (BioTek Cytation3)

In the purification steps, due to the lower affinity, the protein detaches and runs off the column as the column volume is increased