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2.5.4 CBF analysis CBF was analysed using a system consisting of a high-speed acquisition camera (Sony XCD V60, Tokyo, Japan) connected to an Olympus BX51 microscope with a 5 objective (Tokyo, Japan) and a specific software package (Sony ZCL Viewer, Tokyo, Japan), as previously described ( 2.5.5 MCC analysis MCC was monitored using a high-speed acquisition camera (Sony XCD-U100CR, Tokyo, Japan) connected to a microscope with a with a 5 objective (Olympus BX51), as previously described ( 2.5.6 Mucin secretion measurement Mucin production was quantified using an enzyme-linked lectin assay, following a protocol previously published ( 2.5.7 Mucin-5AC detection analysis The presence of goblet cells was assessed through immunohistochemistry analysis for mucin-5AC protein (Muc5AC) detection

doi: 10.3390/ijms21082958 158 TangRDingXZhuJ