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Description

For certain primary antibodies (namely: anti-Fancc, anti-H2AX, anti-Idh1, anti-Sod2, and anti-XPD) sections underwent heat-mediated antigen retrieval and were incubated at 80 C in 10 mM citrate buffer (pH 6.0) for 30 minutes

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Sections (5 m thick) were prepared for subsequent experiments

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Rosenbaum, M., Schrder, U

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