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Baking as a high temperature/short time process (usually 290300 C for 4250 s) had a minimal effect on folate content

Barcode plasmid pool preparation To generate the bacterial CloneSelect barcode library for the Zeocin resistance marker, a semi-random oligonucleotide pool KI#405 encoding 5-ATGCCGVNNVNNVNNVNNVNNTAA-3 was chemically synthesized (Supplementary Table 2)