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The effect of adding a synthetic serum to cultured embryos was similar to that of adding fetal calf serum [61], and the serum did not cause lipid accumulation or organelle damage to bovine embryonic cells [62], reducing the concentration of fetal bovine serum to 5% for culturing in vitro embryonic cells is an appropriate concentration [63]

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( b ) Clustering of time course expression patterns in neurons from mouse brains using the fuzzy c-means algorithm

Notably, elevated intestinal permeability has been implicated in IBD (Yu et al., 2022), celiac disease (Gundry, 2020) and Type 1 diabetes (Miele et al., 2020)