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Le Dr Luc Montagnier et ses collaborateurs ont rapidement compris que le VIH dtriorait le systme immunitaire en raison dune rplication virale massive et du dommage cellulaire caus aux lymphocytes. En parallle, le Dr Wulf Drge, premier vice-prsident directeur, recherche et dveloppement chez Immunotec, et ses collgues sont devenus les premiers chercheurs dcouvrir (en 1989) que des patients infects par le VIH prsentaient des concentrations de cystine et de glutathion (GSH) anormalement basses

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14 PCR amplification was performed in a final volume of 25 l of the reaction mixture containing 1.5 U of Taq DNA polymerase (Sibenzyme), about 1 g DNA of each patient's DNA, 0.25 M each primer, 250 M of dNTPs, 2 mM of MgCl 2 , 1 PCR buffer of the following composition: 67 mM Tris-HCl pH 8.8, 16.6 mM (NH 4 ) 2 SO 4 and 0.01% Tween-20

doi: 10.1073/pnas.050004797, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] Holtzman D